Werner syndrome ATP-dependent helicase; DNA helicase; RecQ-like type 3; RecQ3; Exonuclease WRN; 31--; RecQ protein-like 2; WRN; RECQ3; RECQL2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This WRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 787-816 amino acids from the Central region of human WRN.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-WRN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the RecQ subfamily and the
DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA
helicases are involved in many aspects of DNA metabolism, including
transcription, replication, recombination, and repair. This protein
contains a nuclear localization signal in the C-terminus and shows
a predominant nucleolar localization. It possesses an intrinsic 3'
to 5' DNA helicase activity, and is also a 3' to 5' exonuclease.
Based on interactions between this protein and Ku70/80 heterodimer
in DNA end processing, this protein may be involved in the repair
of double strand DNA breaks. Defects in this gene are the cause of
Werner syndrome, an autosomal recessive disorder characterized by
premature aging.

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

WB~~1:1000

WRN Antibody (Center T802) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the WRN antibody detected the WRN protein (arrow).

WRN Antibody (Center T802) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

162461

Werner syndrome, RecQ helicase-like

Werner syndrome ATP-dependent helicase

Werner syndrome ATP-dependent helicase

RECQ3; RECQL2; RecQ3??[Similar Products]

WRN_HUMAN

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008]

WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating s for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.

Protein type: Nucleolus; DNA repair, damage; EC 3.6.4.12; DNA-binding; Helicase; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: MutLalpha complex; nucleoplasm; centrosome; nucleolus

Molecular Function: G-quadruplex DNA binding; protein homodimerization activity; ATPase activity; magnesium ion binding; 3'-5' DNA helicase activity; bubble DNA binding; helicase activity; four-way junction helicase activity; ATP-dependent DNA helicase activity; Y-form DNA binding; protein binding; DNA helicase activity; ATP-dependent 3'-5' DNA helicase activity; DNA binding; manganese ion binding; exonuclease activity; protein complex binding; 3'-5' exonuclease activity; ATP binding

Biological Process: nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; multicellular organismal aging; cell aging; replicative cell aging; cellular response to starvation; response to UV-C; DNA duplex unwinding; replication fork processing; DNA recombination; regulation of apoptosis; DNA synthesis during DNA repair; base-excision repair; double-strand break repair; regulation of growth rate; response to oxidative stress; DNA replication; DNA metabolic process; telomere maintenance; response to DNA damage stimulus; aging

Disease: Werner Syndrome

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Perry, J.J., et al. J. Biol. Chem. 285(33):25699-25707(2010)
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Kobayashi, J., et al. Mech. Ageing Dev. 131(6):436-444(2010)
Ehrenberg, M., et al. Mol. Vis. 16, 1771-1775 (2010) :

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