Product Name
EIF1AX, siRNA
Full Product Name
EIF1AX siRNA (Human)
Product Synonym Names
EIF1A; EIF4C; Eukaryotic translation initiation factor 1A. X-chromosomal; eIF-1A X isoform; Eukaryotic translation initiation factor 4C; eIF-4C
Product Gene Name
EIF1AX sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P47813
Specificity
EIF1AX siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EIF1AX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of EIF1AX sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
EIF1AX sirna
siRNA to inhibit EIF1AX expression using RNA interference
Applications Tested/Suitable for EIF1AX sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for EIF1AX. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001403.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001412.3
[Other Products]
UniProt Primary Accession #
P47813
[Other Products]
UniProt Secondary Accession #
Q0VGC2; Q5JPS5; Q5JPS6; B2R5U5[Other Products]
UniProt Related Accession #
P47813[Other Products]
Molecular Weight
16,460 Da
NCBI Official Full Name
eukaryotic translation initiation factor 1A, X-chromosomal
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 1A, X-linked
NCBI Official Symbol
EIF1AX??[Similar Products]
NCBI Official Synonym Symbols
EIF1A; EIF4C; eIF-1A; eIF-4C; EIF1AP1
??[Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 1A, X-chromosomal
UniProt Protein Name
Eukaryotic translation initiation factor 1A, X-chromosomal
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 4C; eIF-4C
Protein Family
Eukaryotic translation initiation factor
UniProt Gene Name
EIF1AX??[Similar Products]
UniProt Synonym Gene Names
EIF1A; EIF4C; eIF-1A X isoform; eIF-4C??[Similar Products]
UniProt Entry Name
IF1AX_HUMAN
NCBI Summary for EIF1AX
This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq, Jul 2008]
UniProt Comments for EIF1AX
EIF1AX: Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. Belongs to the eIF-1A family
Protein type: Translation; RNA-binding; Translation initiation
Chromosomal Location of Human Ortholog: Xp22.12
Cellular Component: cytosol
Molecular Function: protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity
Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression
Research Articles on EIF1AX
1. Then, activated p38 further enhanced the docking of activating transcription factor 4 (ATF4) onto the CHOP (CCAAT/enhancer-binding protein homologous protein) promoter via eIF2alpha to enhance apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
