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產(chǎn)品名稱: Gateway LR Cloning Kit
產(chǎn)品型號: E085
產(chǎn)品展商: 其他品牌
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Gateway LR Cloning Kit
Gateway LR Cloning Kit
的詳細介紹
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Print Version
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| Description |
The Gateway cloning system from abm is a flexible cloning method that allows for easy subcloning of one Entry Clone into a wide variety of destination vectors. Gateway technology makes it easy to study your gene of interest using many different promoters and tags. It eliminates the need for restriction enzyme digests or ligations by harnessing the natural ability of phage λ to integrate its DNA into E. coli DNA at specific attachment (att) sites. Gateway reaction recombinations are conservative, directional, and always maintain reading frame. The Gateway system uses both positive selection (antibiotic resistance) and negative selection (toxin-producing ccdB gene) during transformation, ensuring a high proportion of successful recombinants.
Use abm’s BP Clonase Mix (Cat. E082/E083) with a pDONR vector and your gene flanked by attB sites to create your own Entry Clone, or purchase an ORF Vector from abm’s extensive library. It’s then quick and easy to insert your gene of interest into any destination vector using our LR Clonase Mix (Cat. E084/E085). |
| Application |
Easily clone your gene from one Entry Clone into many expression systems with a variety of promoters and tags. No dependence on restriction enzyme digests or ligations.Maintains reading frame and orientation.Fast and Easy.Less expensive than competitors. |
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