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Custom AAV plasmid cloning without compatible insert

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產(chǎn)品名稱: Custom AAV plasmid cloning without compatible insert
產(chǎn)品型號: C235
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

Custom AAV plasmid cloning without compatible insert


Custom AAV plasmid cloning without compatible insert  的詳細(xì)介紹
Description
Among all viral vectors, recombinant Adeno-Associated Virus (AAV) is the most promising candidate for gene therapy owing to its non-integrating nature and lack of pathogenicity animal models. Other key attributes making AAV a very attractive tool in regenerative medicine are its broad tropism and the ability to transduce both proliferating and quiescent cells. Differential transduction and tissue distribution of AAV can be achieved through the use of different AAV serotype capsids. We now also offer custom AAV siRNA vectors and viruses.

Advantages of AAV:

  • Do not elicit significant immune responses in vivo
  • Broad tropism - tissue specificity with different AAV serotypes
  • No integration into the host genome
  • Ability to transduce both proliferating and quiescent cells
  • Long-term expression in non-dividing cells
abm offers custom AAV production with serotypes 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10. The tropism of AAV serotype is summarized in the following table:
AAV Serotype Tissue Specificity (X= Recommended Application)
CNS/Retina Heart Lung Liver Skeletal Muscle
AAV1 X X X - X
AAV2 X - - X X
AAV3 X X - X -
AAV4 X X - - -
AAV5 X - X - -
AAV6 - X X X X
AAV7 X - - X X
AAV8 X - - X X
AAV9 X X X X X
Service Selection Assistant & Services List

How are you going to provide your insert?



What titer would you like to produce?



What serotype would you like to use?



To order custom services please contact us. To aid your selection of the service option(s), please use the following
Gene Synthesis
Product Name Size Cat. No. Price
Gene SynthesisPer bp C098 $0.18
*minimum charge $99; maximum size 3kb for AAV
Custom AAV Plasmid Cloning
Product Name Size Cat. No. Price
Custom AAV plasmid cloning with compatible insert1 Service C234 $350.00
Custom AAV plasmid cloning without compatible insert 1 Service C235 $450.00
*For these services, the customer must provide a gene insert under 3kb. Please contact technical@abmgood.com for more information on available cloning vectors and restriction enzyme sites to qualify for the compatible insert service.
Custom AAV Production without DNA Amplification
Product Name Size Cat. No. Price
Custom AAV without DNA amplification (10^9 GC/ml) (AAV1)5 x 200 μl C239 $550.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV2)5 x 200 μl C240 $550.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV5)5 x 200 μl C241 $550.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV6)5 x 200 μl C242 $550.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV7)5 x 200 μl C243 $550.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV8)5 x 200 μl C244 $550.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV9)5 x 200 μl C245 $550.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV10)5 x 200 μl C246 $550.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV1)2 x 100 μl C247 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV2)2 x 100 μl C248 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV5)2 x 100 μl C249 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV6)2 x 100 μl C250 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV7)2 x 100 μl C251 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV8)2 x 100 μl C252 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV9)2 x 100 μl C253 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV10)2 x 100 μl C254 $950.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV1)2 x 100 μl C255 $2,500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV2)2 x 100 μl C256 $2,500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV5)2 x 100 μl C257 $2,500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV6)2 x 100 μl C258 $2,500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV7)2 x 100 μl C259 $2,500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV8)2 x 100 μl C260 $2,500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV9)2 x 100 μl C261 $2,500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV10)2 x 100 μl C262 $2,500.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV3)5 x 200 μl C290 $550.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV4)5 x 200 μl C291 $550.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV3)2 x 100 μl C292 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV4)2 x 100 μl C293 $950.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV3)2 x 100 μl C294 $2,500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV4)2 x 100 μl C295 $2,500.00
*To qualify for Custom AAV Production without DNA amplification, the following amounts of the AAV vector containing the gene of interest must be supplied at >0.5μg/μl: 10^9 GC/ml: 75 μg, 10^12 GC/ml: 600 μg, 10^13 GC/ml: 1.5 mg.
Custom AAV Production with DNA Amplification
Product Name Size Cat. No. Price
Custom AAV with DNA amplification (10^9 GC/ml) (AAV1)5 x 200 μl C263 $750.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV2)5 x 200 μl C264 $750.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV5)5 x 200 μl C265 $750.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV6)5 x 200 μl C266 $750.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV7)5 x 200 μl C267 $750.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV8)5 x 200 μl C268 $750.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV9)5 x 200 μl C269 $750.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV10)5 x 200 μl C270 $750.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV1)2 x 100 μl C271 $1,150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV2)2 x 100 μl C272 $1,150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV5)2 x 100 μl C273 $1,150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV6)2 x 100 μl C274 $1,150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV7)2 x 100 μl C275 $1,150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV8)2 x 100 μl C276 $1,150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV9)2 x 100 μl C277 $1,150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV10)2 x 100 μl C278 $1,150.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV1)2 x 100 μl C279 $2,700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV2)2 x 100 μl C280 $2,700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV5)2 x 100 μl C281 $2,700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV6)2 x 100 μl C282 $2,700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV7)2 x 100 μl C283 $2,700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV8)2 x 100 μl C284 $2,700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV9)2 x 100 μl C285 $2,700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV10)2 x 100 μl C286 $2,700.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV3)5 x 200 μl C296 $750.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV4)5 x 200 μl C297 $750.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV3)2 x 100 μl C298 $1,150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV4)2 x 100 μl C299 $1,150.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV3)2 x 100 μl C300 $2,700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV4)2 x 100 μl C301 $2,700.00
*To qualify for Custom AAV Production with DNA amplification, 10μg of the AAV vector containing the gene of interest must be supplied, along with a plasmid map and full sequence for plasmid amplification.
Custom siRNA AAV Production
Product Name Size Cat. No. Price
Custom AAV siRNA Pooled Vector1.0 μg iAAV04400 $395.00
AAV siRNA Pooled Virus (AAV1)5 x 200 μl iAAV04401 $795.00
AAV siRNA Pooled Virus (AAV2)5 x 200 μl iAAV04402 $795.00
AAV siRNA Pooled Virus (AAV3)5 x 200 μl iAAV04403 $795.00
AAV siRNA Pooled Virus (AAV4)5 x 200 μl iAAV04404 $795.00
AAV siRNA Pooled Virus (AAV5)5 x 200 μl iAAV04405 $795.00
AAV siRNA Pooled Virus (AAV6)5 x 200 μl iAAV04406 $795.00
AAV siRNA Pooled Virus (AAV7)5 x 200 μl iAAV04407 $795.00
AAV siRNA Pooled Virus (AAV8)5 x 200 μl iAAV04408 $795.00
AAV siRNA Pooled Virus (AAV9)5 x 200 μl iAAV04409 $795.00
AAV siRNA Pooled Virus (AAV10)5 x 200 μl iAAV04410 $795.00
Titer Upgrades for Existing AAV Viruses
Product Name Size Cat. No. Price
Upgrade from 10^9 AAV Virus to High Titer AAV Virus (10^12 GC/ml)2 x 100 μl C288 $400.00
Upgrade from 10^9 AAV Virus to High Titer AAV Virus (10^13 GC/ml)2 x 100 μl C289 $1,900.00
AAV Cell Line
Product Name Size Cat. No. Price
AAV Packaging Cell Line for High Titer Virus Production10^6 Cells C236 $250.00
MCF7-M Cell Line for Infectivity Assay10^6 Cells C237 $250.00
Custom AAV Titration
Product Name Size Cat. No. Price
Custom AAV Titration using qPCR Assay1 Service C238 $75.00
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