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產(chǎn)品名稱: T4 DNA Polymerase
產(chǎn)品型號: E005
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
T4 DNA Polymerase
T4 DNA Polymerase
的詳細介紹
|
Print Version |
| Description |
T4 DNA Polymerase catalyzes the 5’→3’ synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3’→5’ exonuclease activity but lacks 5’→3’ exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3’-overhangs or 5’-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis. |
| Application |
Generates blunt end DNA by filling in 5'-overhangs or/and removing 3'-overhangs
High fidelity due to strong 3’→5’ exonuclease activity
Synthesis of labeled DNA probes by the replacement reaction
Site-specific mutagenesis via primer extension from oligonucleotides |
| Form |
Enzyme supplied with 10X Reaction Buffer |
| Image |
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| Concentration |
5 U/μl |
| Storage |
Store all components at -20°C. |
| Storage Buffer |
100 mM KPO4 (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol. |
| Notes |
One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use. |
