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產(chǎn)品名稱: Phi29 DNA Polymerase
產(chǎn)品型號: E014
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Phi29 DNA Polymerase
Phi29 DNA Polymerase
的詳細(xì)介紹
|
Print Version |
| Description |
Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates. |
| Application |
? Rolling circle amplification (RCA)
? Multiple displacement amplification (MDA)
? Whole genome amplification (WGA)
? DNA template preparation for sequencing
? Protein-primed DNA amplification |
| Form |
Enzyme supplied with 5X Reaction Buffer |
| Image |
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| Concentration |
10 U/μl |
| Storage |
Store all components at -20°C. |
| Storage Buffer |
50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5% Tween-20, 0.5% NP-40 and 50% (v/v) Glycerol. |
| Notes |
One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use . |
