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產(chǎn)品名稱: T4 DNA Ligase
產(chǎn)品型號: G467
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
T4 DNA Ligase
T4 DNA Ligase
的詳細介紹
|
Print Version |
| Description |
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini. |
| Application |
? Cloning of restriction fragments
? Attaching double-stranded oligonucleotide linkers or adaptors to DNA
? Site-directed mutagenesis
? Nick repair of duplex DNA, RNA or DNA-RNA hybrids
? Self-circularization of linear DNA |
| Form |
Enzyme supplied with 5X Reaction Buffer |
| Image |
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| Concentration |
1 U/μl |
| Storage |
Store all components at -20°C. |
| Notes |
This product is distributed for laboratory research only. Caution: Not for diagnostic use. |
