5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)
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產(chǎn)品名稱: 5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)
產(chǎn)品型號: G492
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)
5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)
的詳細介紹
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Print Version |
| Description |
Removal of contaminating genomic DNA from your samples to reverse transcription of RNA in 30 minutes, with 5X All-In-One RT MasterMix (AccuRT Genomic DNA Removal Kit included). abm’s 5X All-in-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) offers a convenient and complete system to facilitate the complete elimination of contaminating genomic DNA in RNA preparations for rapid synthesis of high-quality cDNA for downstream applications.
Key Features
? Efficient ds and ssDNA digestion in under 10 minutes
? Maximal RNA protection – No heating or organic extraction steps required
? Fewer handling errors, with only 1 liquid transfer step for reverse transcription
? Streamlined protocol suitable for high-throughput applications
? Simple set-up for any RNA template
? High reproducibility and excellent cDNA yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup. |
| Literature |
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| Application |
? Generation of templates for use in RT-PCR and qRT-PCR
? cDNA synthesis from ssRNA
? cDNA library construction
? Generation of probes for hybridization
? DNA primer extension |
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| Protocol |
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| Storage |
Store all components at -20C |
| Notes |
This product is ISO-13485 certified. |
