2X PCR SensTaq HotStart MasterMix
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產(chǎn)品名稱: 2X PCR SensTaq HotStart MasterMix
產(chǎn)品型號(hào): G939
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
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2X PCR SensTaq HotStart MasterMix
2X PCR SensTaq HotStart MasterMix
的詳細(xì)介紹
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Print Version |
| Description |
SensTaq HotStart DNA Polymerase is a new type of thermal stable DNA polymerase that is fused with a dsDNA binding subunit. This feature allows the DNA polymerase to stay binding on the template for longer, thus improving its sensitivity and processivity. The enzyme is also an antibody-based hotstart DNA polymerase which gives the enzyme a very good specificity. The proprietary antibody blocks polymerase activity at low temperatures. During the initial denaturation step, the antibody dissociates from the polymerase and restores enzyme activity. Therefore SensTaq is an ideal choice for detection assays and limited template situation such as single-cell target amplification. SensTaq HotStart DNA Polymerase has 5’ to 3’ exonuclease activity but lacks 3’ to 5’ proofreading activity, and it is able to amplify products up to 1.5kb in length containing a single base (A) overhang on the 3’ ends. |
| Literature |
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| Application |
? Genotyping
? Colonies screening
? RT-PCR
? Detection assay
? Single-cell cDNA amplification |
| Protocol |
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| Storage |
Stored at -20°C and protect from light. |
