Description

Affinity chromatography is a method of separating biochemical mixtures based on highly specific biological interactions. The Protein A agarose beads are developed for purification of classes, subclasses and fragments of immunoglobulins, especially IgGs of different species. Protein A is a cell wall component isolated from Staphylococcus aureus that contains five antibody-binding domains with affinity for IgG, some IgA and IgM. This high affinity domain-Fc region of the immunoglobulins interaction makes one-step purification possible for proteins under native conditions. The immunoglobulins bind to Protein A, which are immobilized onto a solid support. Impurities are removed and the purified immunoglobulins can be eluted by decreasing the pH.

Applications of Protein A Agarose Beads

Purification of a wide range of immunoglobulins of different mammalian species.

Purification of certain IgG subclasses that have no affinity

Assays involving protein-protein interactions.

Structural and functional investigation of antibodies.

Special Features

One-step easy purification protocol from crude lysate.

Purification under native conditions.

Can be used for successive cycles.

Ready-to-use for any scale of purification.

Protein binding capacity: approx. >25mg human IgG/ml gel.

Resins and columns formats suitable for batch and gravity purification.