BrightGreen miRNA qPCR MasterMix-iCycler
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產(chǎn)品名稱: BrightGreen miRNA qPCR MasterMix-iCycler
產(chǎn)品型號: MasterMix-mC
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
BrightGreen miRNA qPCR MasterMix-iCycler
BrightGreen miRNA qPCR MasterMix-iCycler
的詳細介紹
|
Print Version |
| Description |
BrightGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples. The components of BrightGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase. |
| Form |
MasterMix |
| Protocol |
 |
| Concentration |
2X |
| Storage |
Upon arrival, BrightGreen miRNA qPCR MasterMix should be stored at -20oC and protected from light. After each experiment, the leftover thawed mix can be stored at 4oC if it is to be used within the next 3 months. |
| Notes |
Primer concentration should not be high. 100nM to 300nM each
- primer usually gives best results
- Melting curve is useful in distinguishing nonspecific amplification products and primer dimers from desired product. It's recommended to run melting curve following amplification cycles.
- To do replicates
- To reduce pipetting error: preparing amplicon specific master mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range are very effective to get tight Ct.
This product is ISO-13485 certified. |
