BrightGreen 2X qPCR MasterMix-No Dye
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產(chǎn)品名稱: BrightGreen 2X qPCR MasterMix-No Dye
產(chǎn)品型號(hào): MasterMix-S
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
BrightGreen 2X qPCR MasterMix-No Dye
BrightGreen 2X qPCR MasterMix-No Dye
的詳細(xì)介紹
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Print Version |
| Description |
Guaranteed high-performance real-time PCR using abm’s BrightGreen 2X qPCR MasterMix. abm’s BrightGreen 2X qPCR MasterMix provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Available with the option of ROX or fluorescein as the internal passive reference dye, abm’s BrightGreen 2X qPCR MasterMix offers unparalleled performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers.
Key Features
? Antibody hot-start technology provides superior specificity
? Enable streamlined protocol in a simple reaction set-up
? Allow accurate quantification of a variety of gene targets
? Reduce pipetting steps to minimize the risk of contamination
? Compatible with most real-time PCR instruments
Please refer to our qPCR MasterMix Selection Guide (found in the Documents section below) for selecting the appropriate qPCR formulation applicable to your particular instrument model. |
| Application |
? Gene-expression analysis
? Gene knockdown validation
? Microarray validation
? High throughput applications
? Virus detection and quantification |
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| Protocol |
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| Storage |
Stored at -20°C and protect from light. |
| Notes |
1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.
2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.
This product is ISO-13485 certified. |
