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產(chǎn)品名稱: T7 RNA Polymerase
產(chǎn)品型號: E041
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
T7 RNA Polymerase
T7 RNA Polymerase
的詳細(xì)介紹
|
Print Version |
| Description |
T7 RNA Polymerase is a DNA dependent RNA polymerase that catalyzes the synthesis of RNA in the 5′→ 3′ direction only in the presence of its cognate T7 phage promoter sequence. T7 RNA Polymerase has high specificity for the T7 phage promoter and will not recognize SP6 or T3 RNA Polymerase promoter sequences. |
| Components |
Enzyme supplied with 10X Reaction Buffer |
| Application |
? Synthesis of RNA transcripts for hybridization probes
? Synthesis of RNA for in vitro translation
? Synthesis of biologically active mRNA
? Generate large amounts of labelled or non-labelled RNA |
| Image |
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| Concentration |
50 U/ul |
| Storage |
Store all components at -20°C. |
| Storage Buffer |
50 mM Tris-HCl (pH 8), 1 mM EDTA, 20 mM β-ME, 100 mM NaCl, 0.1% Triton?X-100 and 50% (v/v) Glycerol. |
| Notes |
One unit is defined as the amount of T7 RNA Polymerase that is required to incorporate 1 nmol ATP into acid-insoluble material in a 50 μl reaction volume in 1 hour at 37°C in 1X T7 RNA Polymerase Reaction Buffer. |
