Poly(A) Polymerase, Yeast
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產(chǎn)品名稱(chēng): Poly(A) Polymerase, Yeast
產(chǎn)品型號(hào): E017
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無(wú)相關(guān)文檔
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Poly(A) Polymerase, Yeast
Poly(A) Polymerase, Yeast
的詳細(xì)介紹
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Print Version |
| Description |
Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli. |
| Components |
Enzyme supplied with 5X Reaction Buffer. |
| Application |
? Labelling of RNA with ATP or cordycepin
? Poly(A) tailing of RNA for cloning or affinity purification
? Increasing translation of RNA transferred into eukaryotic cells |
| Image |
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| Concentration |
1 U/μl |
| Storage |
Store all components at -20°C. |
| Storage Buffer |
20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton? X-100 and 50% (v/v) Glycerol. |
| Notes |
One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.
This product is distributed for laboratory research only. Caution: Not for diagnostic use . |
