Immortalized Human Endometrial Stromal Cells (HESC)
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產(chǎn)品名稱: Immortalized Human Endometrial Stromal Cells (HESC)
產(chǎn)品型號(hào): T0533
產(chǎn)品展商: 其他品牌
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Immortalized Human Endometrial Stromal Cells (HESC)
Immortalized Human Endometrial Stromal Cells (HESC)
的詳細(xì)介紹
|
Print Version |
| BioSafety Level |
II |
| Organism |
Homo sapiens |
| Source Organ |
Endometria |
| Growth Properties |
Adherent |
| Morphology |
Fibroblast-like |
| Recommended Seeding Density |
Recommended split ratio of 1:3 to 1:6 |
| Markers |
IGFBP-1, PRL, TF, PAI-1 at decidualization endpoints |
| Applications |
For Research Use Only |
| Immortalization Method |
Serial passaging and transduction with retroviruses expressing hTERT from the supernatant of pA317-hTERT cell line |
| Description |
Decidualization of the endometrium, mainly induced by progesterone, is a process of endometrial remodelling where the endometrial stromal cells differentiate into secretory decidual cells in preparation for pregnancy. Decidualized endometrial stromal cells are characterized by release of prolactin (PRL), insulin-like growth factor binding protein-1 (IGFBP-1), tissue factor (TF), plasminogen activator inhibitor-1 (PAI-1), and expression of basal lamina components such as laminin, collagen IV, and fibronectin.
The Immortalized Human Endometrial Stromal Cells (HESC) is unique in that 1) it displays normal karyotype and 2) it responds to hormone stimulation and retains the morphological pattern and biochemical endpoints of decidualization after treatment of estradiol and medroxyprogesterone, making this cell line a valuable tool in endometrium homeostasis and female reproductive studies. |
| Procedure Overview |
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| Propagation |
Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available from ABM (TM004). To make the complete growth medium, add the following components to the base medium: 10% charcoal-stripped fetal bovine serum (TM998) and Penicillin/streptomycin. Atmosphere: air: 95%, CO2: 5%; Temperature: 37.0°C.
Stimulation of HESC can be done using 10-8 M estradiol (E2) and/or 10-7 M medroxyprogesterone acetate (MPA). |
| Preservation |
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase. |
| Quality Control |
1) TRAPezeELISA was used to detect the telomerase activity in the immortalized cells; 2) ELISA and RT-PCR were performed to detect IGFBP-1, FN, PRL and PAI-1 secretion and mRNA expression; 3) Western blot and densitometry were conducted to determine the expression of TF and Fas/Fas ligand. |
| Disclaimer |
1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
