MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells
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產(chǎn)品名稱: MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells
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簡單介紹
MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells
MITC Inactivated Rat Embryonic Fibroblast (REF) Feeder Cells
的詳細介紹
|
Print Version |
| Safety |
BioSafety Level II |
| Organism |
Rat |
| Source Organ |
Embryo |
| Growth Properties |
Adherent |
| Morphology |
Fibroblast-like |
| Applications |
For Research Use Only. The inactivated REFs have been tested to be significantly better than inactivated MEF in supporting human iPSC reprogramming and long-term undifferentiated proliferation of human iPS cells and human ground state naive pluripotent stem cells. There is no need to change medium at weekend if human iPS or ES cells are split on Friday and cultured on this REF feeder line in iPSC Culture Medium (TM020). |
| Propagation |
The base medium for this cell line is Prigrow IV medium available from ABM (TM004). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 15%; 0.1 mM Non-Essential Amino Acids; and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO2: 5%; Temperature: 37.0°C. |
| Storage |
-180°C |
| Shipping |
On Dry Ice |
| Notes |
For Research Use Only, not for therapeutic or diagnostic purposes. |
