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產(chǎn)品名稱:
EOS Lentivirus (Other)
產(chǎn)品型號:
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
EOS Lentivirus (Other)
EOS Lentivirus (Other)
的詳細(xì)介紹
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Print Version |
| Description |
This ready-to-use lentivirus is part of abm’s Lentiviral Expression System and can be used to overexpress your gene of interest in a wide range of host cells. Lentiviruses have the ability to integrate into the host genome and generate a stable cell line expressing your gene of interest. |
| Procedure Overview |
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| Gene Insert |
EOS
|
| Vector |
PL-SIN-EOS-C(3)-EiP |
| Vector Size |
8482 |
| Insert Size |
0 bp |
| Tags |
No Tags |
| Selection Marker |
Puromycin |
| Format |
Virus |
| Appearance |
Liquid |
| Storage Buffer |
Serum-free DMEM with 5% PEG6000 |
| Stability |
Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Disclaimer |
1. Disclaimer for transcript variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final.
2. Disclaimer for gene sequence: The provided accession number refers to the transcript (mRNA) sequence for this product. Please verify that this is the desired transcript sequence by cross-referencing. This is important because a single gene can have multiple different transcripts owing to naturally occurring variations. All sales are final.
3. Disclaimer for intended use: All of abm's vectors and viral particles are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its vector(s) in therapeutic/diagnostic application(s).
4. Disclaimer for extra nucleotides: Cloning may lead to the insertion of extra nucleotides at the 5' or 3' end of the gene of interest which, in most cases, is innocuous to the stability/functionality and the expression of that gene.
5. Disclaimer for the stop codon: The stop codon for the inserted gene is located after the 3' cloning site in our GIII vectors. Our GIII vectors contain open ORF inserts (no stop codon) to allow for subsequent insertion into other vectors with C-terminal tags or C-terminal fusion proteins if desired. Please note that a stop codon will need to be added by PCR to the end of the gene insert if no tag or fusion protein is needed in subsequent vectors.
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| Guarantee |
abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data to evaluate the level of gene expression. The replacement will not be covered by the same guarantee.
Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions. |
