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OCT4 Lysate

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產(chǎn)品名稱: OCT4 Lysate
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OCT4 Lysate


OCT4 Lysate  的詳細(xì)介紹
Print Version
Organism Human
Accession # Q01860
Alias POU5F1, OCT3, OTF3
Protein Type Wild Type
Protein Sequence HHHHHHAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICKAETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNRRQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFPEGEAFPPVSVTTLGSPMHSNESGGGGSPGRRRRRRRRR
Protein Family Belongs to the POU transcription factor family. Class-5 subfamily.
Functions ? Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). OCT4 forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. OCT4 is critical for early embryogenesis and for embryonic stem cell pluripotency.
? OCT4 is one of the four factors in both the Thomson and the Yamanaka protocol for iPSC reprogramming. The recombinant protein within the lysate has a 6X His tag at N-terminus for purification and 9X Arginine at C-terminus for cell membrane penetration.
Cell Types Expressed in developing brain.
Protein Location Nucleus.
Tag 6X His tag at N-terminus and 9X Poly-Arginine at C-terminus
Form Liquid
Expression System E.coli
Type Lysate
Purification None
Quality Control By Western Blot Analysis
Concentration 1mg/ml
Storage DMEM with 2.5% glycerol
Shipping On dry ice.
Notes For Research Use Only, not for therapeutic or diagnostic purposes.
References Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20.

Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26.

Induction of pluripotent stem cells from ***** human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72.

Induction of pluripotent stem cells from mouse embryonic and ***** fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.
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