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產(chǎn)品名稱: OCT4 Purified Protein
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簡單介紹
OCT4 Purified Protein
OCT4 Purified Protein
的詳細介紹
|
Print Version |
| Organism |
Human |
| Accession # |
Q01860 |
| Alias |
POU5F1, OCT3, OTF3 |
| Protein Type |
Wild Type |
| Protein Sequence |
HHHHHHAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICKAETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNRRQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFPEGEAFPPVSVTTLGSPMHSNESGGGGSPGRRRRRRRRR |
| Protein Family |
Belongs to the POU transcription factor family. Class-5 subfamily. |
| Functions |
? Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). OCT4 forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. OCT4 is critical for early embryogenesis and for embryonic stem cell pluripotency.
? OCT4 is one of the four factors in both the Thomson and the Yamanaka protocol for iPSC reprogramming. The recombinant protein within the lysate has a 6X His tag at N-terminus for purification and 9X Arginine at C-terminus for cell membrane penetration. |
| Cell Types |
Expressed in developing brain. |
| Protein Location |
Nucleus. |
| Tag |
6X His tag at N-terminus and 9X Poly-Arginine at C-terminus |
| Form |
Liquid |
| Expression System |
E.coli |
| Type |
Purified Protein |
| Purification |
Affinity Chromatography |
| Quality Control |
By Western Blot Analysis |
| Concentration |
250ug/ml |
| Storage |
PBS |
| Shipping |
On dry ice. |
| Notes |
For Research Use Only, not for therapeutic or diagnostic purposes. |
| References |
Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20.
Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26.
Induction of pluripotent stem cells from ***** human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72.
Induction of pluripotent stem cells from mouse embryonic and ***** fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10. |
