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產(chǎn)品名稱: REF-iPSC
產(chǎn)品型號(hào):
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
REF-iPSC
REF-iPSC
的詳細(xì)介紹
|
Print Version |
| Safety |
BioSafety Level II |
| Organism |
Rat |
| Source Organ |
Embryo |
| Concentration |
106cells/vial |
| Propagation |
The optimized medium for this cell line is Rat iPSC Culture Medium available from ABM (TM021). This cell line needs to be cultured on inactivated REF feeder cells (T2017). Atmosphere:air: 95%, CO2: 5%; Temperature: 37.0°C. |
| Subculturing |
1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new feeder cell culture vessel. 6. Incubate cultures at 37°C. Be sure to use inactivated REF feeder cells (T2017). |
| Storage |
-180°C |
| Shipping |
On Dry Ice |
| Notes |
For Research Use Only, not for therapeutic or diagnostic purposes. |
