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產(chǎn)品名稱: 3Dye? 2D DIGE Kits
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3Dye? 2D DIGE Kits
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3Dye? 2D DIGE Kits
Lumiprobe 3Dye? 2D DIGE Kits are specially designed for precise comparison of protein expression in two or three samples of lysates. Perfect mobility matching and bright dye fluorescence allows for high efficient detection and high resolution of minor proteins on 2D gel electrophoresis.
2D DIGE Gel containing Cy2, Cy3, and Cy5 labeled proteins
The kits are compatible to all imagers capable of detection of Cy2, Cy3, and Cy5 dyes. The gels labeled with Lumiprobe 2D DIGE Kits are ready for subsequent mass-spectrometrical analysis.
Each 3Dye? 2D DIGE kit contains reactive NHS esters of Cyanine2, Cyanine3, and Cyanine5 dyes, and labeling grade dimethylformamide (DMF) solvent. Cyanine2 can be used as a reference which can be added to both samples.
Reactive dyes are contained in lyophilized form, which prolonges shelf-life of the kits. Each tube contains exactly the same quantity of reactive dyes (variation within 10%). Re-constitution of the NHS esters with included DMF guarantees that labeling efficiency of Cyanine2, Cyanine3, and Cyanine5 remains the same during entire term of kit use.
Please order kit components separately:
Cy2 fluor (5 nmol, 10 nmol, or 25 nmol)
Cy3 fluor (5 nmol, 10 nmol, or 25 nmol)
Cy5 fluor (5 nmol, 10 nmol, or 25 nmol)
DMF labeling grade solvent (1 mL)Lumiprobe 3Dye 2D DIGE Kits are alternative to dyes produced by GE Healthcare.
