Catalogue number
CYT-201
Synonyms
Erythropoietin-Alpha, EPO-a, EPO-alpha, Epoetin, EP, MGC138142.
Introduction
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Description
Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 36-40kDa. EPO-a is purified by proprietary chromatographic techniques.
Source
Chinese Hamster Ovary Cells(CHO).
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Each mg of lyophilized powder contains 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid.
Solubility
It is recommended to reconstitute the lyophilized Epoetin-a in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR.
Biological Activity
The Specific Activity was measured by Normocyth -aemic mice and was found to be 150,000 IU/mg.
References
1.Title:Zinc Transporters ZnT1 (Slc30a1), Zip8 (Slc39a8), and Zip10 (Slc39a10) in Mouse Red Blood Cells Are Differentially Regulated during Erythroid Development and by Dietary Zinc Deficiency1–3
Publication:Journal of Nutrition, doi:10.3945/jn.108.093575
Vol. 138, No. 11, 2076-2083, November 2008
? 2008 American Society for Nutrition J. Nutr. 138:2076-2083, November 2008
Link:http://jn.nutrition.org/content/138/11/2076.full
2.Title:Systemically delivered Erythropoietin transiently enhances ***** hippocampal neurogenesis.
Publication:Article first published online: 7 MAY 2007 DOI:10.1111/j.1471-4159.2007.04684.x Journal of Neurochemistry? Volume 102, Issue 6, pages 1953–1965, September 2007.
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2007.04684.x/full
3.Title:ZINC TRANSPORTER EXPRESSION IN MATURE RED BLOOD CELLS AND
DIFFERENTIATING ERYTHROID PROGENITOR CELLS.
Publication:UNIVERSITY OF FLORIDA
? 2007 Moon-Suhn Ryu
Link:http://ufdcimages.uflib.ufl.edu/UF/E0/02/14/46/00001/ryu_m.pdf
4.Title:Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury.
Publication:First Published Online May 3, 2012 doi: 10.5966/sctm.2011-0048 Stem Cells Trans Med May 2012 vol. 1 no. 5 373-383
Link:http://stemcellstm.alphamedpress.org/content/1/5/373.full
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
