Catalogue number
ANT-540
Synonyms
IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF,
HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth
factor, CTL differentiation factor, CDF, IL-6, HGF.
Type
Mouse Anti Human Monoclonal.
Introduction
Il-6 is a cytokine with a wide variety of
biological functions: it plays an essential role in the final differentiation of b-cells into ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.
Clone
PAT1F10AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human IL-6, clone
PAT1F10AT mAb, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human IL-6 protein 30-212 amino acids purified from E. coli.
Ig Subclass
Mouse
IgG1 heavy chain and k light chain.
Purification Method
IL-6 antibody was
purified from mouse ascitic fluids by protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
