Ribosomal protein S19, DBA, DBA1, S19, 40S ribosomal protein S19, RPS19.

Ribosomal Protein S19, also known as RPS19, is a part of the S19E family of ribosomal proteins. RPS19 is located in the cytoplasm and required for pre-rRNA processing and maturation of 40S ribosomal subunits. Mutations in RPS19 trigger Diamond-Blackfan anemia, a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients.

RPS19 Human Recombinant produced in E. coli is a single polypeptide chain containing 168 amino acids (1-145) and having a molecular mass of 18.4kDa. RPS19 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered clear solution.

The RPS19 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol and 2mM DTT.

Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Greater than 85% as determined by SDS-PAGE.

MGSSHHHHHH SSGLVPRGSH MGSMPGVTVK DVNQQEFVRA LAAFLKKSGK LKVPEWVDTV KLAKHKELAP YDENWFYTRA ASTARHLYLR GGAGVGSMTK IYGGRQRNGV MPSHFSRGSK SVARRVLQAL EGLKMVEKDQ DGGRKLTPQG QRDLDRIAGQ VAAANKKH.

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