Catalogue number
                            ANT-647 
                                Synonyms
                                NNE, PPH, MPB1, MBP-1, ENO1L1, ENO1, Alpha-Enolase, Enolase-Alpha, 2-phospho-D-glycerate hydro-lyase, Non-neural enolase, Enolase 1, MPB-1, Phosphopyruvate hydratase, C-myc promoter-binding protein, Plasminogen-binding protein, MBPB1.
                                Type
                                Mouse Anti Human Monoclonal. 
                                Introduction
                                ENO1 is a homodimeric soluble protein that encodes a smaller monomeric structural lens protein, tau-crystallin. ENO1 is a glycolytic enzyme expressed in mainly all tissues. ENO1 isoenzyme full length protein is found in the cytoplasm. The shorter protein is formed from another translation start that is restricted to the nucleus, and binds to a component in the c-myc promoter. ENO1 is involved in anaerobic metabolism under hypoxic conditions and plays a role as a cell surface plasminogen receptor during tissue invasion. Irregular expression of Enolase-1 is linked with tumor progression in several cases of breast and lung cancer. Enolase-1 is as an auto antigen associated with Hashimoto's encephalopathy and severe asthma. ENO1 is the target protein of serum anti-endothelial antibody in Behcet's disease. 
                                Clone
                                PAT1G7AT.
                                Physical Appearance
                                Sterile filtered colorless solution.
                                Immunogen
                                Anti-human ENO1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ENO1 amino acids 1-434 purified from E. coli.
                                Ig Subclass
                                Mouse IgG2a heavy chain and k light chain.
                                Purification Method
                                ENO1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
                                Formulation
                                1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
 
                                Storage Procedures
                                For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
 
                                Stability / Shelf Life
                                12 months at -20°C. 1 month at 4°C. 
                                Applications
                                ENO1 antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
                                Safety Data Sheet
                                
                                Usage
                                ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.