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Ca2+ channel R-type, α-1E subunit - 152 403

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產(chǎn)品名稱: Ca2+ channel R-type, α-1E subunit - 152 403
產(chǎn)品型號(hào): 152 403
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Ca2+ channel R-type, α-1E subunit - 152 403


Ca2+ channel R-type, α-1E subunit - 152 403  的詳細(xì)介紹
Cat. No. 152 403 50 μg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to AA 1921 to 2222 from rat Ca2+ channel R-type α-1E (UniProt Id: Q07652)
Reactivity Reacts with: rat (Q07652), mouse (Q61290).
Other species not tested yet.
Specificity Specific for Ca2+ channel α-1E.
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen.
This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended:

IHC: This antibody requires mild fixation. recommended protocol

Datasheet 152_403.pdf
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