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Ca2+ channel N-type, α-1B subunit - 152 305

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產(chǎn)品名稱(chēng): Ca2+ channel N-type, α-1B subunit - 152 305
產(chǎn)品型號(hào): 152 305
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Ca2+ channel N-type, α-1B subunit - 152 305


Ca2+ channel N-type, α-1B subunit - 152 305  的詳細(xì)介紹
Cat. No. 152 305 50 μg specific antibody, lyophilized. Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization. For reconstitution add 50 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
EM: yes gallery  
Immunogen Recombinant protein corresponding to AA 2056 to 2336 from rat Ca2+ channel N-type α-1B (UniProt Id: Q02294)
Reactivity Reacts with: rat (Q02294), mouse (O55017).
Other species not tested yet.
Specificity Specific for Ca2+ channel α-1B.
matching control protein/peptide 152-3P
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen.
This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

IHC: This antibody requires mild fixation. recommended protocol

Datasheet 152_305.pdf
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