Ca2+ channel P/Q-type, α-1A subunit - 152 103
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產(chǎn)品名稱: Ca2+ channel P/Q-type, α-1A subunit - 152 103
產(chǎn)品型號: 152 103
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Ca2+ channel P/Q-type, α-1A subunit - 152 103
Ca2+ channel P/Q-type, α-1A subunit - 152 103
的詳細介紹
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Cat. No. 152 103
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50 μg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
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Applications
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WB: 1 : 1000 (see remarks) gallery
IP: not tested yet
ICC: 1 : 500 up to 1 : 5000 gallery
IHC: yes (see remarks)
IHC-P/FFPE: not tested yet
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Immunogen
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Recombinant protein corresponding to AA 856 to 888 from mouse Ca2+ channel P/Q-type α-1A (UniProt Id: P97445)
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Reactivity
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Reacts with: rat (P54282), mouse (P97445).
Other species not tested yet.
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Specificity
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Specific for Ca2+ channel α-1A. (K.O. verified)
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Remarks
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WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen.
This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: Antibody 2, cat. no. 152 203, is highly recommended for this application.
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Datasheet
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152_103.pdf
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