Ca2+ channel P/Q-type, α-1A subunit - 152 211
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產品名稱: Ca2+ channel P/Q-type, α-1A subunit - 152 211
產品型號: 152 211
產品展商: 其他品牌
產品文檔: 無相關文檔
簡單介紹
Ca2+ channel P/Q-type, α-1A subunit - 152 211
Ca2+ channel P/Q-type, α-1A subunit - 152 211
的詳細介紹
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Cat. No. 152 211
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100 μg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
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Applications
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WB: 1 : 1000 (AP staining) (see remarks) gallery
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
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Clone
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23F11
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Subtype
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IgG2b (κ light chain)
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Immunogen
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Recombinant protein corresponding to AA 1921 to 2212 from rat Ca2+ channel P/Q-type α-1A (UniProt Id: P54282)
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Epitop
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Epitop: AA 1921 to 2212 from rat Ca2+ channel P/Q-type α-1A (UniProt Id: P54282)
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Reactivity
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Reacts with: rat, mouse.
Other species not tested yet.
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Specificity
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Specific for Ca2+ channel α-1A.
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Remarks
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WB: Less sensitive than the polyclonal antibodies. Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen.
This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody requires mild fixation (1-2% PFA for 15min).
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Datasheet
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152_211.pdf
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