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VGLUT 2 - 135 403

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產品名稱: VGLUT 2 - 135 403
產品型號: 135 403
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VGLUT 2 - 135 403


VGLUT 2 - 135 403  的詳細介紹
Cat. No. 135 403 50 μg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 10000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 250 up to 1 : 1000 gallery  
IHC-P/FFPE: 1 : 500 gallery  
ELISA: yes (see remarks)
Immunogen Recombinant protein corresponding to AA 510 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: human (Q9P2U8), rat (Q9JI12), mouse (Q8BLE7), chicken.
Other species not tested yet.
Specificity Specific for VGLUT 2.
matching control protein/peptide 135-4P
Remarks

WB: VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 135 411 as capture antibody (protocol for sandwich-ELISA).
This antibody is highly recommended as marker for glutamatergic nerve terminals and gives excellent results in ICC.
Datasheet 135_403.pdf
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