Goat-anti-mouse Fab fragments - 701-msCpH
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產(chǎn)品名稱: Goat-anti-mouse Fab fragments - 701-msCpH
產(chǎn)品型號(hào): 701-msCpH
產(chǎn)品展商: 其他品牌
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Goat-anti-mouse Fab fragments - 701-msCpH
Goat-anti-mouse Fab fragments - 701-msCpH
的詳細(xì)介紹
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Cat. No. 701-msCpH
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50 μg purified Fab1 fragment, lyophilized, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization. For reconstitution add 50 μl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
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Applications
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ICC: yes (see remarks) gallery
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Label
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CypHer5E
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Remarks
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The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment.
ICC: Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 μg of primary antibody with 5 μg of Fab fragments) in a total volume of 10 μl for 30 min at RT in the dark.
Adjust the antibody to the desired dilution and immediately use it for your internalization assay.
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Datasheet
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701-mscph.pdf
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