Goat-anti-rabbit Fab fragments - 702-rbCpH
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產(chǎn)品名稱: Goat-anti-rabbit Fab fragments - 702-rbCpH
產(chǎn)品型號(hào): 702-rbCpH
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
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Goat-anti-rabbit Fab fragments - 702-rbCpH
Goat-anti-rabbit Fab fragments - 702-rbCpH
的詳細(xì)介紹
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Cat. No. 702-rbCpH
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50 μg affinity purified Fab fragments, lyophilized. Rabbit serum albumin was added for stabilization. For reconstitution add 50 μl H2O to get a 1mg/ml solution of antibody in PBS.
Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt!
Avoid bright light to minimize photo bleeching of the fluorescent dye.
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Applications
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ICC: yes (see remarks) gallery
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Label
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CypHer5E
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Remarks
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Antigen-purified primary antibodies are recommended. Unpurified rabbit serum or total IgG preparations may contain considerable amounts of unspecific IgGs that will bind plenty of the rbFab-CpH resulting in a lower overall labelling efficiency.
The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment.
ICC: Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 μg of primary antibody with 5 μg of Fab fragments) in a total volume of 10 μl for 30 min at RT in the dark.
Adjust the antibody to the desired dilution and immediately use it for your internalization assay.
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Datasheet
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702-rbcph.pdf
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