mCLING-ATTO 488-labeled - 710 006AT3
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產(chǎn)品名稱: mCLING-ATTO 488-labeled - 710 006AT3
產(chǎn)品型號(hào): 710 006AT3
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無(wú)相關(guān)文檔
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mCLING-ATTO 488-labeled - 710 006AT3
mCLING-ATTO 488-labeled - 710 006AT3
的詳細(xì)介紹
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Cat. No. 710 006AT3
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5nmol mCLING labeled with ATTO® 488 in 100 μl PBS (lyophilized). For reconstitution add 100 μl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.
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Applications
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ICC: 1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml) gallery
IHC: 1 : 25 up to 1 : 50 (1 - 2 nmol/ml)
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Label
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ATTO 488
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Remarks
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Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.
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Datasheet
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710_006at3.pdf
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