GABA-A receptor α1 - 224 205
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產(chǎn)品名稱: GABA-A receptor α1 - 224 205
產(chǎn)品型號(hào): 224 205
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
GABA-A receptor α1 - 224 205
GABA-A receptor α1 - 224 205
的詳細(xì)介紹
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Cat. No. 224 205
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50 μg specific antibody, lyophilized. Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization. For reconstitution add 50 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
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Applications
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WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery
IP: not tested yet
ICC: 1 : 200 up to 1 : 1000 (see remarks) gallery
IHC: 1 : 500 (see remarks) gallery
IHC-P/FFPE: not tested yet
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Immunogen
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Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
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Reactivity
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Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
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Specificity
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Specific for GABA-A receptor α1. (K.O. verified)
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matching control protein/peptide
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224-2P
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Remarks
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WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents. IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
recommended protocol
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Datasheet
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224_205.pdf
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