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產(chǎn)品名稱: GluN 1 - 114 011
產(chǎn)品型號: 114 011
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
GluN 1 - 114 011
GluN 1 - 114 011
的詳細介紹
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Cat. No. 114 011
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100 μg purified IgG, lyophilized. For reconstitution add 100 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
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Applications
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WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery
IP: yes (see remarks)
ICC: 1 : 1000 (see remarks) gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
ELISA: yes (see remarks)
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Clone
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M68
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Subtype
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IgG2b (κ light chain)
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Immunogen
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Recombinant protein corresponding to AA 660 to 811 from rat GluN1 (UniProt Id: P35439)
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Epitop
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Epitop: AA 660 to 811 from rat GluN1 (UniProt Id: P35439)
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Reactivity
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Reacts with: human (Q05586), rat (P35439), mouse (P35438), zebrafish.
Other species not tested yet.
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Specificity
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Specific for GluN 1.
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Remarks
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IP: For most effective IP use the solubilization protocol described in this ELISA protocol. Consider that protein-protein interaction may be affected. ICC: For proteins of the post-synaptic density (PSD) para-formaldehyde fixation is not recommended. A methanol fixation is more suitable and gives better results; recommended protocol.
This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 114 003 as detector antibody (protocol for sandwich-ELISA).
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Datasheet
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114_011.pdf
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