Complement convadase assay
Complement convadase assay是一種用于確定生物材料補(bǔ)體激活的靈敏且特異性的方法。
在與血漿孵育過程中，補(bǔ)體因子與材料表面結(jié)合，然后形成補(bǔ)體合酶復(fù)合物。 洗滌后，將生物材料在含有特定顯色底物的培養(yǎng)基中孵育，以去除未結(jié)合的補(bǔ)體蛋白。 通過測量 405 nm 的光密度來量化底物裂開。
◆ 應(yīng)用
生物材料誘導(dǎo)的補(bǔ)體激活的測量
◆ 原理
將感興趣的測試物質(zhì)（理想情況下應(yīng)知道表面積）作為血漿孵育。 在孵育過程中，補(bǔ)體因子與材料表面結(jié)合，然后形成補(bǔ)體自相酶復(fù)合物。
孵育后，洗滌樣品并使用補(bǔ)體相容酶特異性顯色底物分析補(bǔ)體相容酶活性。 顯色速率取決于生物材料表面產(chǎn)生的相聚酶的量。
◆ 儲(chǔ)存及穩(wěn)定性
產(chǎn)品在 -20°C 下可穩(wěn)定保存至少 6 周
◆ 參考文獻(xiàn)
1. Fernández-Colino A, Wolf F, Rütten S, Schmitz-Rode T, Rodríguez-Cabello JC, Jockenhoevel S, Mela P. Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering. Front Bioeng Biotechnol. 2019 Nov 19;7:340.
2. Wiegand C, Abel M, Hipler UC, Elsner P, Zieger M, Kurz J, Wendel HP, Stoppelkamp S. Hemostatic wound dressings: Predicting their effects by in vitro tests. J Biomater Appl. 2019 Apr; 33(9):1285-1297.
3. Naso F, Stefanelli U, Buratto E, Lazzari G, Perota A, Galli C, Gandaglia A. Alpha-Gal Inactivated Heart Valve Bioprostheses Exhibit an Anti-Calcification Propensity Similar to Knockout Tissues. Tissue Eng Part A. 2017 Oct; 23(19-20):1181-1195.
4. Aguiari P, Iop L, Favaretto F, Fidalgo CM, Naso F, Milan G, Vindigni V, Spina M, Bassetto F, Bagno A, Vettor R, Gerosa G. In vitro comparative assessment of decellularized bovine pericardial patches and commercial bioprosthetic heart valves. Biomed Mater. 2017 Feb 3; 12(1):015021.
5. Diaz-Gomez L, Alvarez-Lorenzo C, Concheiro A, Silva M, Dominguez F, Sheikh FA, Cantu T, Desai R, Garcia VL, Macossay J. Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation. Mater Sci Eng C Mater Biol Appl. 2014 Jul 1;40:180-8.
6. Gladitz M, Bauer J, Brückner P, Reinemann S, Wiegand C, Zieger M, Reddersen K, Hipler U, Frant M, Liefeith K, Radusch H. Antibacterial polyamides based on a dendritic zinc-hybrid with good biocompatibility showing reduced biofilm formation, BioNanoMaterials, 2014, 15(1-2), 31-46.
7. Jovanovic D, et al. Polyacylurethanes as novel degradable cell carrier materials for tissue engineering. Materials, 2011, 4.10: 1705-1727.
8. Jovanovic D, Engels GE, Plantinga JA, Bruinsma M, van Oeveren W, Schouten AJ, van Luyn MJ, Harmsen MC. Novel polyurethanes with interconnected porous structure induce in vivo tissue remodeling and accompanied vascularization. J Biomed Mater Res A. 2010 Oct; 95(1):198-208.
◆ 產(chǎn)品信息
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產(chǎn)品名稱
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規(guī)格
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貨號(hào)
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品牌
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Complement Convertase Assay Test kit
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30 determinations
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K005
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HaemoScan
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