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產(chǎn)品名稱: Mouse IFN Beta, E.Coli
產(chǎn)品型號: 12400-1
產(chǎn)品展商: PBL Assay Science
產(chǎn)品文檔: 無相關文檔
簡單介紹
重組蛋白 Mouse Interferon Beta 由 PBL Assay Science（目錄號 #12400-1）生產(chǎn)，僅供研究使用 (RUO)，包裝為 1e5 (100,000) 單位/瓶。Mu IFN-Beta 在大腸桿菌中表達。
Mouse IFN Beta, E.Coli
的詳細介紹
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產(chǎn)品介紹
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品牌
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PBL Assay Science
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貨號
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12400-1
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品名
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Mouse IFN Beta, E.Coli
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規(guī)格
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1 x 105 units
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分子量
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19.6 kDa
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來源
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Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992)
"Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" (J. Interferon Res. 12: 139-43).
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純度
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> 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
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生物活性
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Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV.
EC50 for IFNβ is ~ 2.5U/ml
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緩沖液
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20 mM HEPES, pH 6.0; 0.5M NaCl; 6% glycerol; 0.1% bovine serum albumin (BSA)
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內(nèi)毒素
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< 1 EU/μg
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純度
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> 95%
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純化方法
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A combination of ion exchange, hydrophobic interaction and size exclusion chromatography
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貯存
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For retention of full activity store at -70 oC or below and avoid repeated freeze-thaw cycles
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E.coli expressed Mouse IFN Beta protein with a single non-glycosylated polypeptide chain
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Increased stability with engineered disulfide bond
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95% or greater purity
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