Erpetoichthys calabaricus, slender lungfish, Polypterus senegalus, Mongolian jird, human, mouse, rat, zebrafish , Caenorhabditis elegans, rhesus macaque

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

Mouse monoclonal

Conc. supernat. lyophil.

Lyophilized concentrated supernatant (no preservatives). 

each vial with 200 μl of bidistilled water

The antibody against calretinin was produced in mice by immunization with recombinant human calretinin-22k (1). Calretinin 22k is an alternative splice product of the calretinin gene and identical with calretinin up to Arg178. After fusion, hybridoma cells were screened with human recombinant calretinin as target and the clone 6B3 was selected. The antibody 6B3 recognizes an epitope within the first 4 EF-hands domains common to both calretinin and calretinin-22k (1). It was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffin-sections of 4% paraformaldehyde (or 10% buffered formalin) fixed brains b) by immunoenzymatic labelling of immunoblots c) by immunohistochemistry on tissue of calretinin knock-out mice.

Immunohistochemistry: 1:2'000 - 1:5'000. On paraformaldehyde (4%) or formalin-fixed tissue. Cryostate or paraffin-sections. 

Immunoblots: 1:1'000 - 1:2'000. 

For immunohistochemistry and immunoblots the titer was determined by using the avidinbiotin method. We recommend that the optimal dilutions be determined by titration experiments.

After reconstitution freeze in small aliquots (e.g. 1 μl) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

1. Zimmermann, L. and Schwaller B (2002) Monoclonal antibodies recognizes epitopes of calretinin. Cell Calcium 31: 13-25. 

2. Rogers J.H. (1987) Calretinin: a gene for a novel calcium-binding protein expressed principally in neurons. J. Cell Biol. 105:1343-1353. 

3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137-147. 

4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20:1037-1046. 

5. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. PNAS, 96: 5257-5262.