The antibody 235pur was purified with Protein-G from in vitro produced monoclonal antibody 235 directed against calretinin (see product description 235 for further details concerning immunoblot and immunohistochemistry). The antibody concentration is of 0.38 mg/ml in 0.1M phosphate buffered saline, pH 7.3. 

Immunohistochemistry: we suggest using a dilution of 1:10’000 (0.038 ug/ml) for immunostaining with the avidin-biotin method on cryostat or paraffin-sections of paraformaldehyde (4%) or formalin-fixed tissue. 

We recommend that the optimal dilutions be determined by titration experiments.

 For long storage, keep small aliquots of 1-2 μl at - 80°C (or at least - 20°C). For continuous use keep diluted solutions at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing. 

1. Celio M.R. et al., Cell Calcium 9:81-86, 1988 

2. Schwaller B., et al. (1999) Am. J. Physiol. 276. C395-403