human, mouse, rat, pigs

 western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

 Rabbit

 Antiserum lyophil.

 Lyophilized antiserum (no preservatives). 

 with 200 μl bidistilled water

This antiserum was produced against recombinant rat calbindin D-28k (CB). It crossreacts with calbindin D-28k from many other species, including human, monkey, rat, mouse chicken and fish (Fig. 1). In immunoblots it recognizes a single band of approximately 27 -28 kDa. In samples with very high (10-fold higher than CB) concentrations of the related protein calretinin, a larger band (29 - 30 kDa) may appear in Western blots. Thus, depending on the dilution of the antiserum and/or sensitivity of the immunostaining, this other calcium-binding protein could be also revealed. In case of doubt, we suggest to switch to Swant’s monoclonal antibody 300 directed against calbindin D-28k, which does not recognize calretinin.

Immunohistochemistry: 1:10'000 - 1:20'000 with the avidin-biotin method. 

Immunoblots: 1:100 - 1:1'000. We recommend that the optimal dilutions be determined by titration experiments. 

 Make small portions (e.g. 2-5 μl). For long storage, keep at - 80°C (or at least -20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

 1. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493