產(chǎn)品介紹 |
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名稱 |
Guinea pig antiserum against parvalbumin |
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貨號(hào) |
GP72 |
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規(guī)格 |
100 μl |
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產(chǎn)品形式 |
lyophilized antiserum. No preservatives. |
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復(fù)溶 |
with 100 μl of bi-distilled water. |
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克隆性 |
polyclonal |
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反應(yīng)性 |
human |
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宿主動(dòng)物 |
Guinea-pig |
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抗體形式 |
Antiserum lyophil. |
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產(chǎn)品詳情 |
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產(chǎn)品描述 |
This antibody was raised against recombinant mouse parvalbumin. GP72 reacts specifically with parvalbumin on immunoblots of extracts of tissue originating from human, monkey, guinea pig, rabbit, rat, mouse and chicken (Fig. 1). The antibody specifically localizes parvalbumin using free-floating or mounted sections of brain (Fig. 2), kidney and muscles of probably all vertebrates The antiserum does not stain the brain of parvalbumin-KO mice. Spectacular staining found in immunohistochemistry on floating sections of 4% PFAfixed specimens. |
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稀釋 |
Immunohistochemistry: 1:3'000 - 1:10'000, when performed with the avidin-biotin method. Western blots: 1:1’000 to detect parvalbumin in brain extracts. We recommend that the optimal dilutions be determined by titration experiments. |
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貯存 |
For long storage keep small aliquots of the reconstituted antiserum (e.g. 1 μl) at -80°C (or at least at -20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freeze-thaw cycles. |
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參考資料 |
1. *Filice F., Celio, M.R., Szabolcsi V. (2017) JCN, in press |