The antiserum against hpcal4 is produced in rabbits by immunization with recombinant mouse Hpcal4 containing a 6-his tag at the N-terminal. The antibody was evaluated for specificity and potency: a) by Biotin-Avidin labelling of cryostat-, vibratom- and paraffinsections of 4% paraformaldehyde fixed brains and b) by immunoblots. The product is a polyclonal antiserum against Hpcal4 (1), a calcium-binding protein of the sensor EF-hand family related to hippocalcin. This antiserum stains the brain in a characteristic, unique pattern . The antiserum reacts specifically with Hpcal4 in tissue originating from rodents as determined by immunoblots 

Immunohistochemistry: 1:2'000 - 1:5'000, on paraformaldehyde (4%) or formalin-fixed tissue, cryostate or paraffin-sections. 

Immunoblots: 1:1'000 - 1:2'000. 

For immunohistochemistry and immunoblots the titre was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.

Hpcal4 belongs to the class of the “sensor” calcium binding-proteins. It occurs mainly in the brain, in large subpopulations of nerve cells (see also the Allen brain atlas

After reconstitution freeze in small aliquots (e.g. 1 μl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.